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BioShock 2 is a first-person shooter video game developed by 2K Marin and published by 2K Games, and a sequel to the 2007 video game BioShock. It was released worldwide for Microsoft Windows, PlayStation 3 and Xbox 360 on February 9, 2010. Feral Interactive released an OS X version of the game on March 30, 2012. The game is powered by Unreal Engine 2.5.

Set in the fictional underwater dystopian city of Rapture, the game's story takes place eight years after the events of the first BioShock. Assuming control of Subject Delta, a prototype for the Big Daddies, players are tasked to defeat "splicers", the psychotic human population of the city, using the game's weapons, plasmids, and tonics. Plasmids and tonics grant players active and passive powers respectively. The game also introduces a story-driven multiplayer mode called Fall of Rapture, which takes place during Rapture's 1959 civil war.

BioShock 2 was mainly developed by 2K Marin, with 2K Australia, Arkane Studios, and Digital Extremes providing additional support. 2K Boston, formerly Irrational Games, also assisted the game's development, with series creator Ken Levine providing input. The story received major changes over the course of development. Garry Schyman, the composer of the first game, returned to create the score for BioShock 2.

The game received critical acclaim upon release, with praise particularly directed at the game's narrative, art-style, characters, ending and gameplay. The game attracts criticism for having a slow start and being too similar to its predecessor. The game was supported with downloadable content upon launch, and a new single-player campaign, titled Minerva's Den, was released in August 2010. The game sold more than 3 million copies, but did not meet Take-Two Interactive's sales expectations. A third title, BioShock Infinite, developed by Irrational Games, was released in 2013. A remastered version of the game will be released on Microsoft Windows, PlayStation 4 and Xbox One on September 13, 2016, as part of BioShock: The Collection, along with BioShock and BioShock Infinite.